Composition of sea urchin egg homogenate determines its potency to inositol trisphosphate and cyclic ADPRibose-induced Ca²⁺ release

Title: Composition of sea urchin egg homogenate determines its potency to inositol trisphosphate and cyclic ADPRibose-induced Ca²⁺ release
Creator: Jones, Keith T.; Swann, Karl
Relation: Biochemical and Biophysical Research Communications Vol. 360, Issue 4, p. 815-820
Publisher Link: http://dx.doi.org/10.1016/j.bbrc.2007.06.134
Publisher: Academic Press
Resource Type: journal article
Date: 2007
Description: Of the three intracellular Ca²⁺ signalling molecules (InsP₃, cADPR and NAADP)sea urchin egg homogenate has been used in the identification and characterisation of two, cADPR and NAADP. Homogenate is prepared in a Na⁺/Cl⁻ substitute of N-Methyl glucamine (NMG)/gluconate. To determine how media previous termcompositionnext term affects Ca²⁺ release we replaced NMG with various sugars or glycine and found a dramatic improvement in InsP₃ mediated Ca²⁺ release. Conversely the response to cADPR was diminished, whilst NAADP was unaffected. Therefore modifying media previous termcompositionnext term may be an important consideration in using homogenate to study Ca²⁺ release for future studies.
Subject: calcium; NAADP; egg; inositol trisphosphate; phospholipase C; sea urchin; sperm
Identifier: uon:6555
Identifier: http://hdl.handle.net/1959.13/804219
Identifier: ISSN:0006-291X
Language: eng
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